Map created at http://pga.mgh.harvard.edu/web_apps/web_map/start Editor at http://pga.mgh.harvard.edu/web_apps/dna_utilities.html
*** Sites protected by E. coli methylation are not shown in this map *** SpAcc BstX1 Eci1 Bsg1| Drd1 BsmB1 | | || | | GAAGTGTGGAGGGCCAGAAGGATGGTGCAGTCCTGCTCCGCCTACGGCTGCAAGAACCGCTACGACAAGGACAAGCCCGTCTCCTTCCACAAGTTTCCTC 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 CTTCACACCTCCCGGTCTTCCTACCACGTCAGGACGAGGCGGATGCCGACGTTCTTGGCGATGCTGTTCCTGTTCGGGCAGAGGAAGGTGTTCAAAGGAG orf 1 > M V Q S C S A Y G C K N R Y D K D K P V S F H K F P L BstAP BseY1 Bts1 Pvu2 Xmn1 AlwN1 | | | | | TTACTCGCCCCAGCCTTTGTAAGCAGTGGGAGGCAGCTGTTAAAAGGAAAAACTTCAAGCCCACCAAGTACAGCAGCATCTGCTCGGAGCACTTCACCCC 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 AATGAGCGGGGTCGGAAACATTCGTCACCCTCCGTCGACAATTTTCCTTTTTGAAGTTCGGGTGGTTCATGTCGTCGTAGACGAGCCTCGTGAAGTGGGG orf 1 > T R P S L C K Q W E A A V K R K N F K P T K Y S S I C S E H F T P Ssp1 SpAcc Eco57 | Bbs1 | | | | GGACTGCTTTAAGAGGGAGTGCAACAACAAGCTACTGAAGGAGAACGCTGTGCCCACAATATTTCTCTATATCGAGCCACATGAGAAGAAGGAAGACCTG 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 CCTGACGAAATTCTCCCTCACGTTGTTGTTCGATGACTTCCTCTTGCGACACGGGTGTTATAAAGAGATATAGCTCGGTGTACTCTTCTTCCTTCTGGAC orf 1 > D C F K R E C N N K L L K E N A V P T I F L Y I E P H E K K E D L SpAcc Pst1| || GAATCCCAAGAACAGCTCCCCTCTCCTTCACCCCCCGCTTCCCAGGTTGATGCTGCTATTGGGCTGCTAATGCCCCCTCTGCAGACCCCTGATAACCTGT 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 CTTAGGGTTCTTGTCGAGGGGAGAGGAAGTGGGGGGCGAAGGGTCCAACTACGACGATAACCCGACGATTACGGGGGAGACGTCTGGGGACTATTGGACA orf 1 > E S Q E Q L P S P S P P A S Q V D A A I G L L M P P L Q T P D N L S Pvu2 BspM1 BfuA1 BciV1 Aar1 Bpm1 SpAcc | Ear1 BamH1 Pst1| BpuE1 | | | | | || | | CGGTTTTCTGTGACCACAATTACACTGTGGAGGATACGATGCACCAGAGGAAGAGGATCCTGCAGCTGGAGCAGCAGGTGGAGAAACTCAGGAAGAAGCT 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 GCCAAAAGACACTGGTGTTAATGTGACACCTCCTATGCTACGTGGTCTCCTTCTCCTAGGACGTCGACCTCGTCGTCCACCTCTTTGAGTCCTTCTTCGA orf 1 > V F C D H N Y T V E D T M H Q R K R I L Q L E Q Q V E K L R K K L Nae1 Ahd1 BseY1 NgoM4 | BpuE1 Xho1 Mlu1| | | | | | || CAAGACGGCCCAGCAGCGGTGCCGGCGGCAGGAGAGGCAGCTCGAGAAGCTCAAGGAAGTCGTCCACTTTCAGAGAGAGAAGGACGACGCGTCCGAGAGG 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 GTTCTGCCGGGTCGTCGCCACGGCCGCCGTCCTCTCCGTCGAGCTCTTCGAGTTCCTTCAGCAGGTGAAAGTCTCTCTCTTCCTGCTGCGCAGGCTCTCC orf 1 > K T A Q Q R C R R Q E R Q L E K L K E V V H F Q R E K D D A S E R GGCTACGTGATCCTACCAAATGACTACTTTGAAATTGTTGAAGTTCCAGCATGAAAAAATGAGATGTGTTAGTGGGACAAGACTATACACCTTCTT 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+------ 696 CCGATGCACTAGGATGGTTTACTGATGAAACTTTAACAACTTCAAGGTCGTACTTTTTTACTCTACACAATCACCCTGTTCTGATATGTGGAAGAA orf 1 > G Y V I L P N D Y F E I V E V P A *Found: Aar1 Ahd1 AlwN1 BamH1 Bbs1 BciV1 BfuA1 Bpm1 BpuE1 BseY1 Bsg1 BsmB1 BspM1 BstAP BstX1 Bts1 Drd1 Ear1 Eci1 Eco57 Mlu1 Nae1 NgoM4 Pst1 Pvu2 SpAcc Ssp1 Xho1 Xmn1 Unique: Aar1 Ahd1 AlwN1 BamH1 Bbs1 BciV1 BfuA1 Bpm1 Bsg1 BsmB1 BspM1 BstAP BstX1 Bts1 Drd1 Ear1 Eci1 Eco57 Mlu1 Nae1 NgoM4 Ssp1 Xho1 Xmn1
Not found:
Aat2 Acc65 Acl1 Afe1 Afl2 Age1 Ale1 Apa1 ApaL1 Asc1 Ase1 AsiS1 Avr2 Bae1a
Bae1b BbvC1 Bcg1a Bcg1b Bcl1 BfrB1 Bgl1 Bgl2 Blp1 BmgB1 Bmr1 Bpu10 Bsa1 BsaB1
BsaXa BsaXb BseR1 BsiW1 Bsm1 BspE1 BspH1 BspLU BsrB1 BsrD1 BsrG1 BssH2 BssS1 BstB1
BstE2 BstZ1 Bsu36 BtgZ1 _Chi Cla1 Dra1 Dra3 Eag1 EcoK EcoN1 EcoR1 EcoRV FCatB
FCatL FCatR FCatP ScFRT Fse1 FspA1 Fsp1 Hind3 Hpa1 I_Ceu Kas1 Kpn1 loxP Mfe1
Msc1 Nar1 Nco1 Nde1 Nhe1 Not1 Nru1 Nsi1 Pac1 PflF1 PflM1 Pme1 Pml1 polyA
PshA1 Psi1 PspOM Pvu1 R4atB R4atL R4atP R4atR Rsr2 Sac1 Sac2 Sal1 SanD1 Sap1
Sbf1 Sca1 SexA1 Sfi1 Sgf1 SgrA1 Sma1 SnaB1 SpDon Spe1 Sph1 Srf1 Stu1 Swa1
T3RNA T7RNA T7Ter PISce Xba1 Xcm1
Excluded by site complexity:
Acc1 Aci1 Afl3 Alu1 Alw1 Apo1 Ava1 Ava2 Ban1 Ban2 Bbv1 BceA1 Bfa1 Bme15
BsaA1 BsaH1 BsaJ1 BsaW1 BseM2 BsiE1 BsiH1 Bsl1 BsmA1 BsmF1 Bsp12 BspCa BspCb Bsr1
BsrF1 BssK1 BstF5 BstN1 BstU1 BstY1 Btg1 Cac8 CviJ1 Dde1 Eae1 EcoO1 Fau1 Fnu4H
Fok1 Hae2 Hae3 Hga1 Hha1 Hinc2 Hinf1 HinP1 Hpa2 Hph1 Hpy99 Hpy1 Hpy3 HpyC3
HpyC4 HpyC5 Mae3 Mbo2 Mnl1 Mse1 Msl1 MspA1 Mwo1 Nci1 Nla3 Nla4 Nsp1 Ple1
PpuM1 Rsa1 Sau3A Sau96 SfaN1 Sfc1 Sml1 Sty1 Taq1 Tat1 Tfi1 Tse1 Tsp45 Tsp50
TspR1